4B0N
Crystal structure of PKS-I from the brown algae Ectocarpus siliculosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-23 |
Detector | ADSC QUANTUM Q4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.320, 83.395, 152.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.400 - 2.850 |
R-factor | 0.21201 |
Rwork | 0.211 |
R-free | 0.23587 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ted |
RMSD bond length | 0.010 |
RMSD bond angle | 1.369 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 3.020 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.100 | 0.330 |
Number of reflections | 18963 | |
<I/σ(I)> | 8.5 | 3.4 |
Completeness [%] | 91.7 | 77.4 |
Redundancy | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 3 MICROLITER DROPS OF PROTEIN AT 7 MG/ML WERE MIXED WITH 1.5 MICROLITERS OF CRYSTALLIZATION BUFFER (0.1 M BICINE PH 8, 18% PEG 6000) USING THE HANGING DROP VAPOR DIFFUSION METHOD AT 19 DEGREES CELSIUS |