4AY9
Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Detector | MARRESEARCH MX-300 |
Spacegroup name | P 1 |
Unit cell lengths | 70.716, 95.478, 95.675 |
Unit cell angles | 60.30, 80.02, 75.35 |
Refinement procedure
Resolution | 47.900 - 2.500 |
R-factor | 0.23683 |
Rwork | 0.235 |
R-free | 0.26901 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xwd |
RMSD bond length | 0.007 |
RMSD bond angle | 1.267 |
Data reduction software | HKL-3000 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.400 |
Number of reflections | 70869 | |
<I/σ(I)> | 15.8 | 3.3 |
Completeness [%] | 97.4 | 95.8 |
Redundancy | 6.4 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1M HEPES PH 7.5, 10% (V/V) ISOPROPANOL AND 20% (W/V) POLYETHYLENE GLYCOL 4000 |