4ATV
STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-27 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 115.760, 99.400, 140.230 |
Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
Resolution | 56.980 - 3.500 |
R-factor | 0.288 |
Rwork | 0.287 |
R-free | 0.31270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PRELIMINARY MODEL |
RMSD bond length | 0.004 |
RMSD bond angle | 0.941 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.000 | 3.540 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.040 | 1.110 |
Number of reflections | 37951 | |
<I/σ(I)> | 22 | 1.4 |
Completeness [%] | 94.6 | 69.5 |
Redundancy | 8.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.8 | 0.1 M SODIUM CITRATE PH 3.8, 0.1 M LIS04 AND 26% PEG 400 WITH 1% FACADE-EM AND 1% HEPTYL-THIOL-B-D-GLUCOSIDE AS ADDITIVES |