4AF9
Crystal Structure of Epithelial Adhesin 1 A domain (Epa1A) from Candida glabrata in complex with Galb1-3Glc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-24 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.640, 104.330, 69.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 1.500 |
| R-factor | 0.15683 |
| Rwork | 0.156 |
| R-free | 0.18576 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.601 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.890 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.660 |
| Number of reflections | 43249 | |
| <I/σ(I)> | 14.6 | 2.6 |
| Completeness [%] | 98.8 | 98.7 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 0.2 M AMMONIUM SULFATE, 0.025 M LACTOSE |
