4ABM

Crystal Structure of CHMP4B hairpin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	37.590, 71.430, 123.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.860 - 1.800
R-factor	0.23043
Rwork	0.228
R-free	0.28517
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.018
RMSD bond angle	0.884
Data scaling software	iMOSFLM
Phasing software	SHELX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.230	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.270	0.410
Number of reflections	30007
<I/σ(I)>	16.3	4.4
Completeness [%]	99.6	100
Redundancy	7.8	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1