4A98
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromoflurazepam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-30 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.400, 268.200, 111.400 |
| Unit cell angles | 90.00, 108.10, 90.00 |
Refinement procedure
| Resolution | 25.470 - 3.610 |
| R-factor | 0.2314 |
| Rwork | 0.231 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vl0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.630 |
| High resolution limit [Å] | 3.610 |
| Rmerge | 0.180 |
| Number of reflections | 67378 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 99.6 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
