4A3V
yeast regulatory particle proteasome assembly chaperone Hsm3 in complex with Rpt1 C-terminal fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-02-12 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 186.660, 186.660, 373.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.350 - 3.800 |
R-factor | 0.1913 |
Rwork | 0.190 |
R-free | 0.20850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Phasing software | MOLREP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 3.910 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.080 | 0.710 |
Number of reflections | 38668 | |
<I/σ(I)> | 21.32 | 2.32 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 15.6 | 11.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 5M SODIUM FORMATE, 0.1M HEPES PH 7.5 |