4A1H
Human myelin P2 protein, K45S mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-01 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.130, 63.610, 84.470 |
Unit cell angles | 90.00, 130.10, 90.00 |
Refinement procedure
Resolution | 19.945 - 2.201 |
R-factor | 0.1973 |
Rwork | 0.194 |
R-free | 0.25180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wut |
RMSD bond length | 0.004 |
RMSD bond angle | 0.766 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | 0.620 |
Number of reflections | 22007 | |
<I/σ(I)> | 11 | 1.6 |
Completeness [%] | 88.0 | 49.2 |
Redundancy | 3.4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | pH 4.5 |