4A0I
Crystal structure of Survivin bound to the N-terminal tail of hSgo1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.650, 70.514, 82.382 |
| Unit cell angles | 90.00, 131.80, 90.00 |
Refinement procedure
| Resolution | 41.066 - 2.605 |
| R-factor | 0.2065 |
| Rwork | 0.204 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qfa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.106 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.550 |
| Number of reflections | 14834 | |
| <I/σ(I)> | 16.5 | 2.4 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100MM TRIS PH 8, 200MM NACL, 20% PEG 6000 |
