483D
CRYSTAL STRUCTURE OF THE SARCIN/RICIN DOMAIN FROM E. COLI 23 S RRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-05-14 |
Detector | ADSC |
Spacegroup name | P 43 |
Unit cell lengths | 29.481, 29.481, 76.353 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.110 |
R-factor | 0.152 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | THE RELATED RAT SARCIN/RICIN STRUCTURE (430D) |
RMSD bond length | 0.029 * |
RMSD bond angle | 0.035 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.120 |
High resolution limit [Å] | 1.110 | 1.110 |
Rmerge | 0.042 | 0.487 |
Number of reflections | 25407 | |
<I/σ(I)> | 35.4 | 2.2 |
Completeness [%] | 97.9 | 84.5 * |
Redundancy | 4.7 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 * | 19 * | 3.0 - 3.2 M (NH4)2SO4, (50 MM KMOPS, 10 MM MGCL2, 10MM MNCL2), 2.5 MG/ML RNA, pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | KMOPS | ||
2 | 1 | 1 | MGCL2 | ||
3 | 1 | 1 | MNCL2 | ||
4 | 1 | 1 | (NH4)2SO4 | ||
5 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5 (mg/ml) | |
2 | 1 | drop | Na-EDTA | 1.0 (mM) | |
3 | 1 | drop | Tris | 10 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 3.0-3.2 (M) | |
5 | 1 | reservoir | potassium MOPS | 50 (mM) | |
6 | 1 | reservoir | 10 (mM) | ||
7 | 1 | reservoir | 10 (mM) |