3ZW1
Structure of Bambl lectine in complex with lewix x antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-07 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 81.440, 81.440, 81.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.720 - 1.600 |
| R-factor | 0.1551 |
| Rwork | 0.153 |
| R-free | 0.18904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zw0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.709 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.720 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.100 |
| Number of reflections | 23162 | |
| <I/σ(I)> | 26.6 | 7.9 |
| Completeness [%] | 96.2 | 82.4 |
| Redundancy | 6.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 28 % PEG 6000, 100MM SODIUM ACETATE PH 5.0, 200MM TRISODIUM CITRATE. 10% ETHYLENE GLYCOL WAD ADDED AS CRYOPROTECTANT PRIOR TO FREEZING |
