3ZVK
Crystal structure of VapBC2 from Rickettsia felis bound to a DNA fragment from their promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-26 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.780, 92.333, 150.495 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.420 - 2.500 |
R-factor | 0.1745 |
Rwork | 0.172 |
R-free | 0.21720 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.280 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER (2.9.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.290 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.560 |
Number of reflections | 39577 | |
<I/σ(I)> | 17.4 | 3.2 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 12% PEG4000, 4% PEG400, 100MM MES PH6.5 | ||
1 | 6.5 | 12% PEG4000, 4% PEG400, 100MM MES PH6.5 |