3ZHI
N-terminal domain of the CI repressor from bacteriophage TP901-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.620, 43.590, 71.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.609 - 1.600 |
| R-factor | 0.1374 |
| Rwork | 0.135 |
| R-free | 0.18260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.164 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.340 |
| Number of reflections | 12874 | |
| <I/σ(I)> | 26.49 | 4.9 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.1M SODIUM CITRATE |
