3ZH7
The structure of crystal form II of Haemophilus influenzae protein E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-30 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.670, 42.490, 56.800 |
Unit cell angles | 90.00, 116.31, 90.00 |
Refinement procedure
Resolution | 28.298 - 2.102 |
R-factor | 0.2035 |
Rwork | 0.201 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.029 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.460 |
Number of reflections | 12867 | |
<I/σ(I)> | 11.94 | 2.03 |
Completeness [%] | 91.4 | 79.8 |
Redundancy | 2.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100 MM BISTRIS PROPANE PH 6.5, 200 MM NAI AND 20% W/V POLYETHYLENE GLYCOL 3350 |