3ZG7
Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2010-02-27 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.584, 127.798, 54.869 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.731 - 1.991 |
R-factor | 0.2 |
Rwork | 0.197 |
R-free | 0.24970 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.146 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AutoSol |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.330 |
Number of reflections | 38493 | |
<I/σ(I)> | 19.2 | 2.5 |
Completeness [%] | 92.7 | 64 |
Redundancy | 4.2 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 20% POLYETHYLENE GLYCOL 3350, 0.1M AMMONIUM TARTRATE (PH 7.0) |