3ZFD
Crystal Structure of the Kif4 Motor Domain Complexed With Mg-AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-04-25 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.718, 62.718, 167.524 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.350 - 1.710 |
R-factor | 0.16763 |
Rwork | 0.166 |
R-free | 0.19483 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hqd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.425 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.350 | |
High resolution limit [Å] | 1.710 | 1.700 |
Rmerge | 0.090 | 0.490 |
Number of reflections | 37057 | |
<I/σ(I)> | 21.1 | 3.4 |
Completeness [%] | 99.3 | 95 |
Redundancy | 11.8 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.5 |