3WOD
RNA polymerase-gp39 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-27 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 294.442, 294.442, 223.255 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.882 - 3.600 |
R-factor | 0.251 |
Rwork | 0.250 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iw7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.265 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 3.600 |
Number of reflections | 129380 |
<I/σ(I)> | 7.1 |
Completeness [%] | 99.5 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 45% tacsimate, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |