3WNY
A simplified BPTI variant with poly Lys amino acid tag (C3K) at the C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-04-28 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.878, 71.440, 57.245 |
| Unit cell angles | 90.00, 92.09, 90.00 |
Refinement procedure
| Resolution | 25.090 - 1.300 |
| R-factor | 0.26139 |
| Rwork | 0.260 |
| R-free | 0.29349 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.141 |
| Data reduction software | XFIT |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.230 | 0.230 |
| Number of reflections | 104028 | |
| Completeness [%] | 99.6 | 99.61 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20-25% PEG4000, 0.1M TrisHCl, 0.2M Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
