3WA8
Crystal structure of M. ruber CasB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.007 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.823, 75.383, 112.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.130 - 2.800 |
R-factor | 0.21012 |
Rwork | 0.208 |
R-free | 0.25883 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.502 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 14829 | |
Completeness [%] | 99.9 | 99 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | PEG5K MME, Tascimate, HEPES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |