3VYN
Crystal structure of Mycobacterium tuberculosis L,D-transpeptidase LdtMt2 N55 truncation mutant (resideus 55-408)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2012-06-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.96422 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.100, 93.150, 75.500 |
Unit cell angles | 90.00, 92.86, 90.00 |
Refinement procedure
Resolution | 48.640 - 2.500 |
R-factor | 0.2198 |
Rwork | 0.220 |
R-free | 0.26140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vyo |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.030 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.149 | 0.497 |
Number of reflections | 29308 | |
<I/σ(I)> | 6.8 | 2.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM Bis-Tis, 200mM MgCl2, 20% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |