3VUT
Crystal structures of non-phosphorylated MAP2K4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2009-06-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.773, 87.362, 118.373 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.500 |
R-factor | 0.33471 |
Rwork | 0.331 |
R-free | 0.40666 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.753 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.068 | |
Number of reflections | 9049 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M ammonium acetate, 22-25%(w/v) PEG3350, 0.1M HEPES-NaOH pH7.0, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |