3VT9
Crystal structures of rat VDR-LBD with W282R mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Collection date | 2008-02-28 |
Wavelength(s) | 0.97800 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.714, 42.050, 41.972 |
Unit cell angles | 90.00, 95.63, 90.00 |
Refinement procedure
Resolution | 35.290 - 2.350 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zlc |
RMSD bond length | 0.004 |
RMSD bond angle | 0.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 10561 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MOPS-Na, sodium formate, PEG 4000, ethyleneglycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |