3VEM
Structural basis of transcriptional gene silencing mediated by Arabidopsis MOM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.640, 85.640, 292.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.959 - 3.200 |
R-factor | 0.2601 |
Rwork | 0.258 |
R-free | 0.29320 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.263 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.6.1_353)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 21107 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1 M Tris, 0.3 M magnesium formate dihydrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |