3V8Y
Structure of apo-glycogenin truncated at residue 270
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.430 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.630, 104.220, 120.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.820 - 2.150 |
| R-factor | 0.20719 |
| Rwork | 0.206 |
| R-free | 0.23887 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.294 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.820 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.056 | 0.252 |
| Number of reflections | 20120 | |
| <I/σ(I)> | 11.7 | 2.9 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 1.0 M ammonium sulate/0.1M sodium phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
