3V6N
Crystal structure of a plant albumin from Cicer Arietinum showing hemagglutination
Replaces: 3S0LExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.246, 73.360, 87.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.200 |
| R-factor | 0.18155 |
| Rwork | 0.179 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lp9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.290 |
| Number of reflections | 23739 | |
| <I/σ(I)> | 3.17 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M sodium cacodylate, pH 6.5, 0.2M sodium acetate, 18% w/v polyethylene glycol 8000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
