3V21
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Experimental procedure
Experimental method | SINGLE WAVELENGT |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-01 |
Detector | MAR555 |
Wavelength(s) | 0.8148 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.880, 115.311, 130.243 |
Unit cell angles | 90.00, 112.50, 90.00 |
Refinement procedure
Resolution | 65.090 - 2.700 |
R-factor | 0.234 |
Rwork | 0.230 |
R-free | 0.26700 |
Structure solution method | MR |
Starting model (for MR) | 1knv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.288 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.094 | 65.090 | 2.850 |
High resolution limit [Å] | 2.326 | 8.540 | 2.700 |
Rmerge | 0.061 | 0.027 | 0.388 |
Total number of observations | 11234 | 53541 | |
Number of reflections | 80299 | ||
<I/σ(I)> | 20.3 | 18.3 | 1.9 |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 4.6 | 4.3 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 293 | 0.1 M Bis-Tris, 0.5% polyvinylpyrrolidone and 16% of PEG400, VAPOR DIFFUSION, temperature 293K, pH 5.5 |