3UYK
Spinosyn Rhamnosyltransferase SpnG complexed with spinosyn aglycone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 54.021, 57.076, 68.100 |
Unit cell angles | 81.98, 74.10, 86.07 |
Refinement procedure
Resolution | 29.830 - 1.700 |
R-factor | 0.19113 |
Rwork | 0.189 |
R-free | 0.23431 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tsa |
RMSD bond length | 0.023 |
RMSD bond angle | 2.256 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (MR) |
Refinement software | REFMAC (version: 5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.830 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 78024 | |
Completeness [%] | 96.9 | 91.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 298.15 | 18% w/v PEG3350, 12.5-13.5% w/v glucose, 1% v/v glycerol, 0.1 M magnesium formate, 0.1 M sodium cacodylate, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 298.15K |