3UTK
Structure of the pilotin of the type II secretion system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0718 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.700, 53.095, 98.735 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.762 - 1.650 |
R-factor | 0.1992 |
Rwork | 0.197 |
R-free | 0.24970 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.918 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.780 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.200 | 0.336 |
Number of reflections | 32440 | |
<I/σ(I)> | 6.9 | 2.6 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 7.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2M ammonium sulfate, 2% PEG 400, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |