3UPS
Crystal structure of iojap-like protein from Zymomonas mobilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 18-ID |
| Synchrotron site | APS |
| Beamline | 18-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.397, 75.397, 66.916 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.1635 |
| Rwork | 0.162 |
| R-free | 0.19260 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.987 |
| Number of reflections | 22680 | |
| <I/σ(I)> | 36.7 | 2.37 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 10.7 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 0.2 M LIthium sulfate, 0.1 M phosphate-citrate, 20% PEG1000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
