3ULE

Structure of Bos taurus Arp2/3 complex with bound inhibitor CK-869 and ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-08
Detector	NOIR-1
Spacegroup name	P 21 21 21
Unit cell lengths	111.166, 128.314, 198.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.223
Rwork	0.223
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	1.300
RMSD bond angle	0.010
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	93950
<I/σ(I)>		2
Completeness [%]	95.2	90.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	277	8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K