3UCK
Coccomyxa beta-carbonic anhydrase in complex with phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-06 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9311 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.059, 74.059, 220.502 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.780 - 2.500 |
R-factor | 0.20817 |
Rwork | 0.205 |
R-free | 0.27514 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ucj |
RMSD bond length | 0.019 |
RMSD bond angle | 1.799 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 22331 | |
Completeness [%] | 98.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 1.6-2.3 M sodium/potassium phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |