3UAL
Crystal Structure of 14-3-3 epsilon with Mlf1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97906 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.980, 81.460, 82.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.270 - 1.800 |
| R-factor | 0.17442 |
| Rwork | 0.172 |
| R-free | 0.22240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2br9 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.909 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.456 |
| Number of reflections | 24289 | |
| <I/σ(I)> | 16.8 | 4.1 |
| Completeness [%] | 97.9 | 97.6 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.1 M Na-Citrate, 35% tert-butanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
