3U9G
Crystal structure of the Zinc finger antiviral protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.25 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 52.894, 52.894, 138.275 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.491 - 1.801 |
R-factor | 0.1929 |
Rwork | 0.191 |
R-free | 0.22820 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.938 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.324 | |
Number of reflections | 21576 | |
<I/σ(I)> | 30.4 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 19.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 15% (w/v) PEG 3350, 0.06M magnesium formate dehydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |