3TRZ
Mouse Lin28A in complex with let-7d microRNA pre-element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 143.820, 143.820, 177.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.660 - 2.900 |
R-factor | 0.1847 |
Rwork | 0.183 |
R-free | 0.21020 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | XDS (autoPROC) |
Data scaling software | SCALA |
Phasing software | BUSTER (2.11.1) |
Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.660 | 2.910 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.071 | 0.586 |
Number of reflections | 41278 | |
<I/σ(I)> | 21.4 | 3.2 |
Completeness [%] | 98.5 | 99.8 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | 0.6M NaH2PO4, 1.4M K2HPO4, pH 7, vapor diffusion, temperature 298K |