3TPA

Structure of HbpA2 from Haemophilus parasuis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.480, 61.020, 150.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.639 - 2.000
R-factor	0.1637
Rwork	0.162
R-free	0.20020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GBPA STRUCTURE FROM H. PARASUIS
RMSD bond length	0.008
RMSD bond angle	1.102
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	34819
<I/σ(I)>		2.57
Completeness [%]	99.7
Redundancy		5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M HEPES pH 7.0, 30% v/v jeffamine ED-2001, VAPOR DIFFUSION, SITTING DROP, temperature 293K