3TLV
The GLIC pentameric Ligand-Gated Ion Channel Loop2-22' oxidized mutant in a locally-closed conformation (LC3 subtype)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.140, 127.756, 160.866 |
| Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
| Resolution | 49.230 - 2.900 |
| R-factor | 0.2161 |
| Rwork | 0.215 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data scaling software | SCALA |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.230 | 3.050 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.071 | 0.677 |
| Number of reflections | 78570 | |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 99.4 | 96.5 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 | 293 | pH 4, VAPOR DIFFUSION, temperature 293K |
