3TBD

Crystal Structure of domain VI and LE1 of human Netrin-G2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.071
Spacegroup name	P 32 2 1
Unit cell lengths	63.134, 63.134, 190.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.270 - 1.800
R-factor	0.178
Rwork	0.175
R-free	0.22700
Structure solution method	SAD
RMSD bond length	0.028
RMSD bond angle	2.272
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	2.440	1.860
High resolution limit [Å]	1.800	2.270	1.800
Number of reflections	40558
Completeness [%]		100	74.8
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	20% PEG 3350, 0.2M Calcium acetate, 0.1M Sodium cacodylate pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K