3T7U
A NeW Crystal structure of APC-ARM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 72.496, 72.496, 129.981 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.2712 |
| Rwork | 0.269 |
| R-free | 0.31260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nmw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.908 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.081 | 0.060 | 0.550 |
| Number of reflections | 10096 | ||
| <I/σ(I)> | 16.5 | ||
| Completeness [%] | 99.1 | 94.1 | 100 |
| Redundancy | 20.3 | 17.8 | 20.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 1.02M Sodium phosphate monobasic monohydrate, 0.18M Potassium phosphate dibasic, vapor diffusion, hanging drop, temperature 287K |
