3T5N

1.8A crystal structure of Lassa virus nucleoprotein in complex with ssRNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795, 1.0
Spacegroup name	P 21 21 2
Unit cell lengths	74.159, 71.737, 56.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.000 - 1.787
R-factor	0.1837
Rwork	0.182
R-free	0.21000
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.998
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	Auto-Rickshaw
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	37.000
High resolution limit [Å]	1.787
Number of reflections	39465
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	295	0.1M Hepes, pH 7.0, 18% PEG 3350, 12.5mM MgCl 2 and 7.5mM NiCl2, VAPOR DIFFUSION, temperature 295K
1	VAPOR DIFFUSION	7	295	0.1M Hepes, pH 7.0, 18% PEG 3350, 12.5mM MgCl 2 and 7.5mM NiCl2, VAPOR DIFFUSION, temperature 295K