3T5D
Crystal structure of Septin 7 in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-08-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99986 |
Spacegroup name | P 61 |
Unit cell lengths | 82.363, 82.363, 210.598 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 71.250 - 3.300 |
R-factor | 0.28696 |
Rwork | 0.285 |
R-free | 0.31826 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.912 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.250 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.392 | |
Number of reflections | 12079 | |
<I/σ(I)> | 3.85 | |
Completeness [%] | 99.1 | 98.9 |
Redundancy | 8.9 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.2M sodium chlorid, 1.1M ammonium sulfate, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |