3T1B
Crystal structure of the full-length AphB N100E variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 268.737, 54.545, 103.840 |
| Unit cell angles | 90.00, 100.95, 90.00 |
Refinement procedure
| Resolution | 48.081 - 2.700 |
| R-factor | 0.1986 |
| Rwork | 0.197 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3szp |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.651 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (AutoMR) |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.922 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 58215 | |
| <I/σ(I)> | 15.64 | 2.24 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1 M sodium citrate, 0.1 M sodium cacodylate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
