3SWV
Crystal Structure of a domain of Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (Brefeldin A-inhibited GEP 2) from Homo sapiens (Human), Northeast Structural Genomics Consortium target id HR5562A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-01 |
Detector | ADSC QUANTUM 4r |
Spacegroup name | P 32 |
Unit cell lengths | 53.939, 53.939, 75.725 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.26200 |
Structure solution method | SAD |
RMSD bond length | 1.180 |
RMSD bond angle | 2.080 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.069 | 0.309 |
Number of reflections | 9252 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 94.8 | 99.8 |
Redundancy | 2.2 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 291 | 15% PEG1000, 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K | |
1 | 5 | 291 | 15% PEG1000, 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |