3SOP
Crystal Structure Of Human Septin 3 GTPase Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-09-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97627 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.244, 150.414, 157.997 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.352 - 2.885 |
R-factor | 0.252 |
Rwork | 0.251 |
R-free | 0.27170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qnr |
RMSD bond length | 0.008 |
RMSD bond angle | 0.906 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.352 | 3.060 | |
High resolution limit [Å] | 2.885 | 8.540 | 2.885 |
Rmerge | 0.123 | 0.036 | 0.620 |
Number of reflections | 14242 | ||
<I/σ(I)> | 11.81 | 35.33 | 2.34 |
Completeness [%] | 98.4 | 99.2 | 91.8 |
Redundancy | 5.02 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 100 mM Bis-Tris, pH 6.2, 200 mM magnesium formate, 18% PEG3350, 5% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |