3SIH
The X-ray crystal structure of poly(ADP-ribose) glycohydrolase (PARG) from Thermomonospora curvata
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-24 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.245, 50.074, 105.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.156 - 1.500 |
R-factor | 0.1858 |
Rwork | 0.184 |
R-free | 0.21400 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.006 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.803 |
High resolution limit [Å] | 1.500 |
Number of reflections | 37023 |
Completeness [%] | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SITTING DROP, VAPOR DIFFUSION | 9 | 10% PEG6000, 0.1 M Bicine, pH 9.0, SITTING DROP, VAPOR DIFFUSION |