3S97
PTPRZ CNTN1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 334.108, 45.957, 78.947 |
Unit cell angles | 90.00, 101.37, 90.00 |
Refinement procedure
Resolution | 33.561 - 2.297 |
R-factor | 0.2033 |
Rwork | 0.201 |
R-free | 0.25450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 3JXF and 1CS6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.056 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.297 | 4.950 | 2.297 |
Rmerge | 0.153 | 0.088 | 0.493 |
Number of reflections | 51002 | ||
<I/σ(I)> | 7.5 | ||
Completeness [%] | 96.4 | 99.6 | 81.3 |
Redundancy | 6.9 | 7.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% (w/v) PEG 1,500, 50 mM Na-cacodylate pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |