3S3X
Structure of chicken acid-sensing ion channel 1 AT 3.0 A resolution in complex with psalmotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.396, 109.442, 127.269 |
| Unit cell angles | 90.00, 119.81, 90.00 |
Refinement procedure
| Resolution | 29.915 - 2.990 |
| R-factor | 0.2192 |
| Rwork | 0.218 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s3w |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.676 |
| Data reduction software | XDS ((VERSION May 10) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.108 | 0.725 |
| Number of reflections | 55842 | |
| <I/σ(I)> | 8.51 | 1.06 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.38 | 3.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 286 | 0.1M sodium acetate pH 5.5, 3 % Glycerol, 3% 1,6-hexanediol, 11 % PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
