3RMW
Crystal Structure of Human Glycogenin-1 (GYG1) T83M mutant complexed with manganese and UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-10-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 56.650, 100.610, 49.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.860 - 1.930 |
R-factor | 0.18674 |
Rwork | 0.184 |
R-free | 0.23735 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q4s |
RMSD bond length | 0.016 |
RMSD bond angle | 1.404 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.850 | 2.030 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.097 | 0.728 |
Number of reflections | 21806 | |
<I/σ(I)> | 10.7 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 25% PEG smears (PEG 6k, 8k, 10k), 0.2M MgCl2, 10% glycerol, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |