3RFW
The virulence factor PEB4 and the periplasmic protein Cj1289 are two structurally-related SurA-like chaperones in the human pathogen Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9780 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.353, 91.595, 61.553 |
Unit cell angles | 90.00, 102.27, 90.00 |
Refinement procedure
Resolution | 39.750 - 2.200 |
R-factor | 0.2362 |
Rwork | 0.234 |
R-free | 0.27630 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.110 |
Data scaling software | SCALA (3.3.9) |
Phasing software | SOLVE (2.13) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.797 | 45.797 | 2.320 |
High resolution limit [Å] | 2.199 | 6.960 | 2.200 |
Rmerge | 0.051 | 0.443 | |
Rmeas | 0.055 | 0.464 | |
Rpim | 0.020 | 0.138 | |
Total number of observations | 5478 | 36924 | |
Number of reflections | 22275 | ||
<I/σ(I)> | 20.3 | 36.8 | 5.7 |
Completeness [%] | 99.5 | 85.4 | 100 |
Redundancy | 11.3 | 8.8 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 280 | 0.1M succinate, 40% v/v Jeffamine, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 280 | 0.1M succinate, 40% v/v Jeffamine, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K |