3RE4
Crystal Structure of Archaeoglobus Fulgidus Rio1 Kinase bound to Toyocamycin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.814, 72.607, 60.561 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 34.943 - 1.997 |
| R-factor | 0.2173 |
| Rwork | 0.215 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.385 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.943 |
| High resolution limit [Å] | 1.997 |
| Number of reflections | 30275 |
| <I/σ(I)> | 5.5 |
| Completeness [%] | 97.1 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 24% PEG 3350, 0.2 M Mg (CH3COO)2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
