3RBB
HIV-1 NEF protein in complex with engineered HCK SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.977032 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.190, 90.880, 169.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.990 - 2.350 |
| R-factor | 0.20589 |
| Rwork | 0.204 |
| R-free | 0.24513 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.329 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.5.0109) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 28573 | |
| <I/σ(I)> | 22.18 | |
| Completeness [%] | 99.5 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Hepes, 8% ethylene glycol, 22% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
